DNA Sequencing means determining the order of the four chemical building blocks - called "bases" - that make up the DNA molecule. The sequence tells scientists the kind of genetic information that is carried in a particular DNA segment. For example, scientists can use sequence information to determine which stretches of DNA contain genes and which stretches carry regulatory instructions, turning genes on or off. In addition, and importantly, sequence data can highlight changes in a gene that may cause disease.
Since the completion of the Human Genome Project, technological improvements and automation have increased speed and lowered costs to the point where individual genes can be sequenced routinely, and some labs can sequence well over 100,000 billion bases per year, and an entire genome can be sequenced for just a few thousand dollars.
Learn the basics and principles of DNA sequencing which includes:
What is DNA sequencing
History and Principles of DNA sequencing
Methods of DNA sequencing
Maxam-Gilbert sequencing procedure
Sanger sequencing or Chain termination method
Next Generation sequencing (NGS)
DNA polymerase
Massive parallel sequencing
Pyrosequencing
Illumina or Ion Torrent NGS
DNA Nanoball
Single Molecule real time sequencing
Sequence detection methods
Application of Molecular Biology in the Real world:
Application of Molecular Biology in Clinical Research
Early detection of a disease
Prenatal Gene therapy
Personalized medicine
Application of Molecular Biology in Forensics
Application of Molecular Biology in Pharmaceuticals
Genetic testing
Rational drug design
Vaccines
Biological Molecules
Nano systems
Functioning of a Molecular Biology lab