Introduction
  • Applications of Next Generation Sequencing
Genetics
  • DNA structure
  • The code in our DNA
  • Reviewing your experience
  • Using the code to make proteins
  • Genes and chromosomes
  • Genetic variation and how it arises
  • Patterns of inheritance: Dominant and Recessive alleles
  • Patterns of inheritance: Codominance and Incomplete Dominance
  • Many genes for a trait
  • Linked inheritance of alleles
Next Generation DNA Sequencing
  • Sanger Sequencing
  • How Next Generation Sequencing works
  • Preparing DNA libraries for NGS
  • The role of adapters in sequencing
  • Overview of Next Generation DNA Sequencing data analysis
  • Types of sequencing
  • Paired reads: sequencing from both ends
  • Types of genetic variants
  • Somatic variants and cancer
  • NGS for personalized cancer treatment
Project: Start Analyzing NGS Data
  • Galaxy: a platform for analysis
  • Getting publicly accessible data
  • FastQ data format
  • Quality check with FastQC 1
  • Quality check with FastQC 2
  • Improving quality with Trimmomatic