- Introduction
- properties of SDS-PAGE
- Gel production
- Sample preparation
- Electrophoresis
- Gel staining
- Applications
- variants and alternatives
What you'll learn
- sodium dodecyl sulphate–polyacrylamide gel electrophoresis technique
Description
Sodium dodecyl sulphate- polyacrylamide electrophoresis technique is used widely in biochemistry, pharmacology, immunology and molecular biology labs. by learning this technique, we can separate any proteins mixtures.
this technique is mainly used for proteins separation.
In this course, We will learn everything about this technique, including preparation of gel and sample.
This course includes main points:
Introduction which includes Sodium dodecyl sulphate- polyacrylamide electrophoresis definition and history technique.
Properties of SDS- PAGE.
The procedure of this system which includes several steps to separate mixture of proteins and applications of this technique.
Finally we recognise the variants and alternatives of this technique.
The gel preparation, sample preparation, electrophoresis and finally gel staining and documentation.
All proteins are sorted by size at the end of the electrophoretic separation and can then be analysed using other methods such as protein staining and immunological detection such as the Western Blot.
I tried to collect all data about sodium dodecyl sulphate polyacrylamide gel electrophoresis technique in these videos in simple way and concentrated to collect much informations and steps in less time.
After finishing this course you know everything about this technique and it become easy to perform it in your lab under scientific considerations.
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About the instructors
- 3.17 Calificación
- 361 Estudiantes
- 2 Cursos
Omnia Ali
Researcher
I am researcher in Biochemistry and Molecular Biology Department in TBRI, Egypt.
I have experience in:
Recombinant proteins expression in prokaryotic systems especially E. coli,cloning and expression of synthetic genes.
Recombinant protein refolding and purification using HPLC and AKTA purifier.
Biochemical characterization of recombinant proteins using sodium dodecyl sulfate poly acrylamide gel, western blot, Bradford assay and ELISA etc.
Protein modification by Pegylation using different PEG derivatives or Nanoencapsulation using chitosan.
Student feedback
Course Rating
Reviews
Good course, with clear and specific information
I found this course really very interesting and it covers all the basic points to learn the protein separation and also the chemistry behind it. In fact, the course is very well structured, concise and elaborative. Thank you very much for such an amzing wonderful effort.